Dual client binding sites in the ATP-independent chaperone SurA

The ATP-independent chaperone SurA protects unfolded outer membrane proteins (OMPs) from aggregation in the periplasm of Gram-negative bacteria, and delivers them to the β-barrel assembly machinery (BAM) for folding into the outer membrane (OM). Precisely how SurA recognises and binds its different OMP clients remains unclear. Escherichia coli SurA comprises three domains: a core and two PPIase domains (P1 and P2). Here, by combining methyl-TROSY NMR, single-molecule Förster resonance energy transfer (smFRET), and bioinformatics analyses we show that SurA client binding is mediated by two binding hotspots in the core and P1 domains. These interactions are driven by aromatic-rich motifs in the client proteins, leading to SurA core/P1 domain rearrangements and expansion of clients from collapsed, non-native states. We demonstrate that the core domain is key to OMP expansion by SurA, and uncover a role for SurA PPIase domains in limiting the extent of expansion. The results reveal insights into SurA-OMP recognition and the mechanism of activation for an ATP-independent chaperone, and suggest a route to targeting the functions of a chaperone key to bacterial virulence and OM integrity.

Bob Schiffrin 1,5 , Joel A. Crossley 1,5 , Martin Walko 1,2 , Jonathan M. Machin 1 , G. Nasir Khan 1 , Iain W. Manfield 1 , Andrew J. Wilson 2,4 , David J. Brockwell 1 , Tomas Fessl 3 , Antonio N. Calabrese 1 , Sheena E. Radford 1 & Anastasia Zhuravleva 1 The ATP-independent chaperone SurA protects unfolded outer membrane proteins (OMPs) from aggregation in the periplasm of Gram-negative bacteria, and delivers them to the β-barrel assembly machinery (BAM) for folding into the outer membrane (OM).Precisely how SurA recognises and binds its different OMP clients remains unclear.Escherichia coli SurA comprises three domains: a core and two PPIase domains (P1 and P2).Here, by combining methyl-TROSY NMR, single-molecule Förster resonance energy transfer (smFRET), and bioinformatics analyses we show that SurA client binding is mediated by two binding hotspots in the core and P1 domains.These interactions are driven by aromatic-rich motifs in the client proteins, leading to SurA core/P1 domain rearrangements and expansion of clients from collapsed, non-native states.We demonstrate that the core domain is key to OMP expansion by SurA, and uncover a role for SurA PPIase domains in limiting the extent of expansion.The results reveal insights into SurA-OMP recognition and the mechanism of activation for an ATP-independent chaperone, and suggest a route to targeting the functions of a chaperone key to bacterial virulence and OM integrity.
The outer membrane (OM) of Gram-negative bacteria is key to cell survival and virulence, providing structural integrity and presenting a barrier to antibiotics, bile salts and detergents 1,2 .The OM bilayer is densely packed with OM proteins (OMPs) [3][4][5] , that form cylindrical βbarrel structures of varying size (8-26 strands in E. coli) [6][7][8] .These proteins perform a variety of functions, including nutrient acquisition, efflux of toxic molecules, and OM assembly, and play critical roles in pathogenesis 8 .OMPs have a complex biogenesis pathway: they are synthesised on cytoplasmic ribosomes and bind to the chaperones Trigger Factor and SecB, before their Sec mediated secretion into the periplasm 9 .Therein, they are maintained in a soluble and foldingcompetent state by periplasmic chaperones (e.g., SurA, Skp, FkpA, and Spy) 10,11 , before being folded into the OM by the essential OMP complex known as the β-barrel assembly machinery (BAM) [12][13][14] .In E. coli, BAM is a ~203 kDa heteropentameric complex, consisting of the conserved subunit BamA, itself a 16-stranded OMP, and four lipoproteins (BamB-E) [15][16][17][18] .
Current evidence suggests that OMPs fold in vivo by sequential formation of β-strands that is templated by the N-terminal β-strand of BamA's β-barrel (β1) making a β-augmentation interaction with the C-terminal β-strand of the incoming OMP substrate 7,[19][20][21][22] .The C-terminal strand in OMPs typically contains a β-signal sequence [ζxGxx[Ω/Φ]x[Ω/Φ] where ζ is a polar residue; Ω is an aromatic residue; Φ is a hydrophobic residue and x is any residue.The β-signal sequence, and in particular its final residue, plays a pivotal role in OMP assembly in vivo 23,24 and in vitro [24][25][26] .Consistent with this model, a recently discovered antibiotic, darobactin, acts as a potent antibacterial by mimicking and inhibiting the OMP: BamA β-augmentation interaction at this site [27][28][29] .This model of BAM-catalysed OMP assembly suggests that chaperones need to deliver unfolded OMPs to BAM for their folding in a vectorial manner, commencing with their C-terminal β-strand.
SurA is thought to be the major OMP chaperone in the periplasm and the primary route for OMP delivery to BAM 30,31 .Deletion of SurA leads to loss of OM integrity, upregulation of the σ E envelope stress response and increased permeability of the OM to large antibiotics, such as vancomycin and rifampicin 30,[32][33][34][35][36] .SurA is also involved in the biogenesis of virulence factors, including adhesins, pili, and autotransporters [37][38][39][40][41][42][43][44][45][46] , and deletion of SurA restores antibiotic sensitivity in a multidrug resistant Pseudomonas aeruginosa strain 47 .In E. coli, SurA contains three domains: a core comprising the N-terminal and C-terminal regions, and two tandem peptidyl prolyl isomerase (PPIase) domains (P1 and P2), only the latter of which is enzymatically functional (Fig. 1a) 48 .The isolated core domain has chaperone activity 49 , and multiple lines of evidence point to functional roles for the P1 and/or P2 domains in facilitating the prevention of OMP aggregation and the delivery of OMPs to BAM 35,41,[49][50][51][52] .Dynamic domain rearrangements between the core and PPIase domains have been proposed to play a role in SurA activity (Fig. 1a, b), although how this disfavours aggregation and promotes delivery to BAM is unclear 50,[53][54][55][56] .
How SurA achieves its specificity for OMP sequences is also unknown, with previous studies indicating a preference for Ar-X-Ar and Ar-Ar containing sequences 57 (where Ar is an aromatic residue and X is any amino acid), or aromatic-enriched sequences containing an Ar-X-Ar-X-Pro motif 58 .Accordingly, the peptide WEYIPNV was shown to bind SurA with µM affinity (K d 1-14 µM) 58 , with X-ray crystallography showing that this peptide binds to the P1 domain 56,59 .This led to a model for SurA-client recognition in which the P1 domain confers SurA's specificity for OMP sequences, with the core domain acting as a more general chaperone 35,59 .Structures of a second aromatic-rich peptide (NFTLKFWDIFRK) bound to the P1 domain (PDB: 2PV2), and to a SurA construct lacking the P2 domain, named SurA-ΔP2 (PDB: 2PV3), support the view that the P1 and/or core domains are important players in SurA-client recognition 59 .It remains unclear, however, whether SurA-client interactions involve a diffuse binding interface between core and P1, or if binding involves specific binding sites, and how SurA inter-domain re-arrangements, that are known to occur upon client binding 56 , mediate its chaperone function.
Here we address these questions using methyl-TROSY NMR, smFRET, binding assays using different OMPs and OMP-derived peptides, and bioinformatic analysis of conservation across SurA sequences.The results reveal that SurA has two conserved client binding hotspots that mediate affinity for unfolded OMP substrates, one in the core domain and one in the P1 domain, and add a distinct dimension to previous models suggesting that SurA binds its clients via a large and diffuse binding interface 54,56 .The discovery of the two binding hotspots provides an opportunity to target these sites with small molecules to attenuate virulence and increase bacterial susceptibility to antibiotics by inhibiting a chaperone key to biogenesis of the OM.

SurA domains tune the conformational properties of a bound OMP client
We began our investigations into the role of the different SurA domains in OMP binding by examining the conservation of PPIase domains in SurA homologues across bacterial species.Using a Crystal structure of wild-type (WT) E. coli SurA (PDB: 1M5Y 48 ) with missing residues added using MODELLER 56,119 .In this structure the P1 domain is bound to the core domain (named here, SurA core-P1 closed).Regions are coloured grey (Nterminal region of the core domain), green (P1), yellow (P2) and orange (C-terminal region of the core domain).This colour scheme for SurA domains is used throughout.b Model of WT SurA in which the P1 domain is extended away from the core domain (named here SurA core-P1 open).The model was built using MODELLER 56,119 and the crystal structures of full-length SurA (PDB: 1M5Y 48 ) and SurA-ΔP2 (PDB: 2PV3  AlphaFold2 (Methods) 60,61 , we determined the number and taxonomy of organisms containing SurA sequences that contain only a core domain, versus those that contain one or more PPIase domains.The analysis revealed that >99% of SurA homologues contain at least one PPIase domain (99.5% of 14,244 homologues) (Fig. 1c), with the few examples of sequences which lack PPIase domains largely restricted to bacteria specialised to extreme environments (Source Data file).This suggests that the presence of at least one PPIase domain is functionally important for bacterial survival and/or virulence.Previous small-angle neutron scattering (SANS) and smFRET experiments have shown that binding to SurA expands its (unfolded) OMP clients 54,62,63 .However, the roles of the different SurA domains in expansion were unknown.To address this question, we used pulsed interleaved excitation smFRET (Supplementary Methods) to monitor the effects of binding SurA-WT, or a construct lacking both PPIase domains (SurA-core) (Fig. 1d), on the conformation and dynamics of the model OMP, OmpX 56,64,65 .Building on recent work 63 , we labelled the N-and C-termini of OmpX with a FRET donor and acceptor dye pair (Methods), and used smFRET to monitor OmpX conformations under different conditions.Fluorescence anisotropy experiments indicated that both dyes at both termini have sufficient rotational freedom to allow changes in FRET values to be assigned to OmpX end-to-end distances (Supplementary Table 1, Supplementary Methods).
Unfolded OmpX diluted from high concentrations of denaturant into low (0.1 M) urea (apo-OmpX) yielded a broad raw FRET distribution (E raw ) with an maximum at ~0.85, suggesting a distribution of conformational species ranging from collapsed to more expanded species in rapid equilibrium (Fig. 2a, upper two panels).Non-randomcoil chain behaviour of unfolded OMPs in low amounts of denaturant is well-established in the field 54,63,66 , as might be expected for an unfolded protein poised to fold once it encounters a bilayer.By contrast, OmpX fully unfolded in 4 M urea resulted in a narrower distribution with a significantly lower E raw maximum (~0.45) (Supplementary Fig. 1d), consistent with a more highly unfolded ensemble, as reported previously 63,66,67 .
When apo-OmpX is bound to SurA-WT (in 0.1 M urea) the smFRET distribution broadens and its maximum is shifted to a lower E max value (~0.7) compared with that of apo-OmpX, consistent with a shift in equilibrium to favour more expanded conformations.Hence, binding to the chaperone results in a net expansion of the collapsed chain, as previously reported 63 (Fig. 2b, upper two panels).Remarkably, the addition of SurA-core shifts the E raw values of OmpX to even lower E max values compared to the protein alone and bound to SurA-WT (E raw maxima of ~0.45), with a concomitant depletion of species with higher apparent E raw values (Fig. 2c, upper two panels).This suggests that the P1 and P2 domains modulate the conformations and dynamics of SurAbound OMP clients.Both SurA-WT and SurA-core have been shown to bind to unfolded OMPs with similar affinity (~μM) and with Hill coefficients > 1 49,50 , suggesting that multiple copies of SurA variants could be bound to each unfolded OmpX chain.One contribution to the greater expansion observed for SurA-core could be additional SurAcore molecules bound to OmpX compared with SurA-WT, although microscale thermophoresis (MST) experiments indicated similar Hill coefficients for the binding of OmpX to both variants, consistent with previous results 50 (Supplementary Fig. 2).
We next investigated the timescales of dynamics of the OmpX chain of the different samples apo-OmpX (in 0.1 M and 4 M urea) and bound to SurA-WT or SurA-core (in 0.1 M urea).Using Burst Variance Analysis (BVA) and corrected FRET efficiency vs normalised fluorescence lifetime we probed for dynamics on ms and faster timescales 68,69  (Methods).Dynamic exchange between species with different E values are observed on both timescales for unfolded apo-OmpX in 0.1 M urea, as well as OmpX bound to SurA-WT and SurA-core (Fig. 2a-c, lower panels, Supplementary Fig. 1).This is consistent with the behaviour expected for a collapsed unfolded chain where dynamics are limited by inter residue interactions in the unfolded-state ensemble 66 .By contrast, ms dynamics are not observed in OmpX in 4 M urea in the BVA analysis (Supplementary Fig. 1d) while faster dynamics are observed as evidenced by the lifetime analysis (Supplementary Fig. 1d), consistent with the behaviour expected for a fully unfolded polymer 66 .
Collectively, the results show that binding to the SurA core domain alone is sufficient to cause a net expansion of unfolded OmpX.In addition, they suggest a role for the PPIase domains in limiting the extent of expansion of the bound client compared to SurA-core, which causes the greatest degree of expansion and lacks these domains.
The SurA core and P1 domains are involved in client binding Previous smFRET experiments have shown that client binding alters the interactions between the P1 and core domains of SurA 56 , although data are currently lacking on precisely where, and how, the different SurA domains interact with their clients and the residues that determine their molecular recognition.To map the client binding regions on SurA in more detail we used site-specific methyl labelling and TROSY NMR.We specifically labelled the δ1 carbon atoms of isoleucine (29 residues) and ε carbon atoms of methionine (14 residues) with 13 C, providing residue-specific probes that span all three domains of SurA (Supplementary Fig. 3a-c) 70,71 .Of the resulting 43 peaks in the spectrum of apo-SurA (i.e., without client), 33 were not overlapped or broadened, and could be assigned unambiguously using mutation (see Methods), providing probes for all three domains (18 in the core, 7 in P1, and 8 in P2).While the majority of peaks have high intensity, three non-overlapped peaks were broadened and of low intensity (M46, I217 and M400) (Supplementary Fig. 3d, e).These three residues are located at, or close to, the core-P1 interface in the crystal structure (Supplementary Fig. 3f, g), suggesting that core-P1 conformational rearrangements occur on an intermediate NMR timescale (~µs-ms) 72 .These conformational rearrangements are likely due to interconversions between the core-P1 closed and open states (Fig. 1a, b), as observed using smFRET 56 and SANS 53 .Analysis of chemical shift changes for the SurA domain deletion variants SurA-ΔP1 (that lacks the P1 domain), SurA-ΔP2 (that lacks the P2 domain) and SurA-core (that lacks both PPIase domains) (Fig. 1d), confirmed that the P1 domain indeed makes extensive interactions with the core, while P2 is more remote (Supplementary Figs. 4, 5).
We next analysed the effect of adding unfolded OmpX on the chemical shift and linewidths of SurA-WT 13 CH 3 resonances using NMR (Fig. 3a-e, Supplementary Fig. 6).Both of these types of peak changes (shifts and/or broadening), can report on binding or conformational changes caused by binding at the location of the corresponding 13 CH 3 probe 73,74 .To ensure a substantial fraction of bound SurA (chosen as ~60% given a K d of ~1 µM 56,63 ) we employed 5 µM concentrations of both SurA and unfolded OmpX.This ensured sufficient binding, and good signal to noise within a few hours data acquisition (Methods), while maintaining an OMP concentration low enough to minimise aggregation 75 .Reductions in the peak intensity of methyl-TROSY resonances of SurA-WT were observed upon the addition of unfolded OmpX, whereas only small chemical shift perturbations (CSPs) were detected (Fig. 3c, Supplementary Fig. 6).Such behaviour (changes in intensity, but only small CSPs) has been observed previously for interactions between other chaperones and their unfolded substrates [76][77][78] , suggesting heterogeneous binding interactions and/or intermediate exchange dynamics.Notably, the intensities of most peaks corresponding to residues in the SurA core domain were affected, consistent with this domain being a major location of OMP interactions 35,49,56 (Fig. 3c).Similarly, 6 out of 7 peaks corresponding to residues in the P1 domain were reduced in intensity by ~50% upon OmpX binding, with one peak (M231) severely affected.By contrast, the peaks representing residues in the P2 domain were much less affected, with small (10-40%) reductions in intensity for all resonances, likely due to the slower molecular tumbling of the chaperone in its client-bound state 79 .We mapped the Z-scores (Methods) 80 of the SurA-WT peak intensity changes in the presence of OmpX (Fig. 3d) onto a model of the chaperone in a core-P1 open state (Fig. 3e).This analysis showed that regions in the core and P1 domains directly interact with OmpX, while P2 is largely uninvolved in OmpX binding, consistent with previous cross-linking mass spectrometry (MS) and hydrogen exchange MS (HDX-MS) data 56 .
Next, to examine the effect of client size and sequence on SurA interactions, we studied the interactions between SurA and different OMPs: tOmpA (19 kDa), OmpF (37 kDa), and tBamA, the transmembrane domain of BamA (43 kDa) (Supplementary Fig. 7a).These clients bind SurA-WT with K d 3.7 ± 1.0 μM, K i 5.2 ± 1.7 µM, and K d 1.4 ± 0.1 μM for tOmpA 50 , OmpF 81 and tBamA (Supplementary Fig. 7b), respectively (the inhibition constant (K i ) for binding of OmpF to SurA was obtained from phage-based ELISA assays 81 ).In each case, the perturbation patterns in NMR resonances were similar to those observed when SurA binds OmpX, with key interactions involving the core and P1 binding sites, and P2 largely uninvolved (Supplementary Fig. 8).

OMP binding hotspots in the core and P1 domains of SurA
While the NMR data presented above suggest that OmpX engages in widespread interactions involving the SurA core and P1 domains, this raises the question of which interaction sites on SurA are key for OmpX recognition and binding affinity.To identify whether, and how, different regions of SurA interact with different residues in the sequence of OmpX, we examined the effect of binding of six short (15 residue) peptides on the NMR spectrum of methyl-labelled SurA-WT.These peptides correspond to regions that form β-strands in native OmpX and also contain Ar-X-Ar or Ar-Ar motifs that have been implicated in SurA binding 57,58,81 (Fig. 3a, b, Supplementary Table 2).While all OmpXderived peptides were found to bind to SurA (Supplementary Figs.9,10), their affinities (K d s > ~100 µM, Supplementary Fig. 11) are more than 100-times weaker than that of full-length OmpX (K d of ~1 µM 56,63 ), implying avidity effects when SurA binds its full length OMP clients.
The addition of the OmpX-derived peptides (200 µM) to SurA (5 µM) also results in substantial changes (intensity reductions and/or CSPs) in the core and/or P1 domains, but with the P2 domain mostly unperturbed (Fig. 3f, g, Supplementary Figs. 9, 10), consistent with the results obtained using full-length OmpX (Fig. 3c, d).However, by contrast to the results for full-length OmpX, peptide binding to the core domain results in the most substantial changes to residues located between the two lobes of the N-domain (residues I91, M109, M114, I132, and M136), with other core domain residues being less, or not affected (Fig. 3h, Supplementary Figs. 9, 10).In the crystal structure of SurA-WT a helix from the core-P1 linker region of a neighbouring molecule in the crystal lattice was found at this location, suggestive of a putative, functional protein-protein interaction site, consistent with our finding that this region is directly involved in client binding (Fig. 3i, Supplementary Fig. 12a).In addition to perturbations to residues in the core domain, the binding of three of the five Ar-X-Ar containing OmpX peptides (QMN (corresponding to the second β-strand of OmpX, named β2), KHD (β6) and SVD (β8), and the Ar-Ar containing peptide NKN (β4)), also affect the peak for M231 in the P1 domain (Fig. 3f-h, Supplementary Figs. 9, 10).This residue is located in the region where the peptide WEYIPNV binds in the crystal structure of the WEYIPNV-P1 complex (Fig. 3i, Supplementary Fig. 12b) 59 .Previous results also showed that diazirine-labelled OmpX forms cross-links to a residue (E248) located close to this site 56 .In the NMR spectrum of SurA-WT bound to WEYIPNV (Supplementary Fig. 13a-h) we also observed severe broadening of the resonance for M231, indicating that this peak is a reporter of direct interaction at this site.Interestingly, patches of high conservation are observed at both the core and P1 domain binding sites determined here (Supplementary Fig. 12c, d), consistent with these interaction hotspots being important for SurA function.
We also noted that the magnitude of changes in peak intensity and CSPs in the SurA core and P1 peptide binding sites varies significantly for the different OmpX peptides.For example, binding to the peptide GVI (OmpX β3) results in significant perturbations in resonances arising from core residues, but only subtle perturbations to those in the P1 binding site (Supplementary Figs. 9, 10).By contrast, binding to NKN (OmpX β4) most severely affects residues in the P1 site and results in smaller perturbations in the core domain (Supplementary Figs. 9, 10).The different peptide sequences, therefore, have different kinetic and/or thermodynamic signatures of binding to the core and P1 SurA binding hotspots, suggesting different (but as yet not fully understood) sequence specificities at each site.

Binding of an OmpX-derived peptide promotes an activated open state
Binding of WEYIPNV to SurA-WT 55,56 , and the gain-of-function mutant SurA-S220A 51 , are known to promote SurA core-P1-open states (Fig. 1b).Residue S220 is located at the core-P1 interface 48 (Supplementary Fig. 14a), and results in the suppression of OM assembly defects caused by BamA/B mutations 55,56 , implying that activation of SurA involves release of the P1 domain from the core domain 52 .Binding of the peptide WEYIPNV to SurA-WT causes substantial CSPs in the P1 domain, as well as the broadening of the M231 peak described above, as expected given the known binding specificity of WEYIPNV for this domain 59 (Fig. 4a, Supplementary Figs. 13, 15).In addition, CSPs are observed for peaks corresponding to residues across the whole core domain, except for residues in the core binding site mapped above using OmpX-derived peptides (Fig. 4a, Supplementary Fig. 13).Identical shifts in these core domain peaks are observed for SurA-S220A, indicating that these peaks report on the same conformational change in both cases (Fig. 4b, Supplementary Fig. 13g), and provide an NMR fingerprint for core-P1 opening.In addition to the changes observed for residues lining the core and P1 binding sites (Supplementary Fig. 9), binding of the OmpX-derived peptide QMN (OmpX β2), resulted in CSPs in identical (or similar) directions to those for the SurA-S220A variant (Fig. 4c, Supplementary Fig. 14).Combined, these results not only demonstrate that QMN peptide binds to SurA-WT in the identified cleft in the core domain, but that binding results in conformational changes associated with core-P1 opening, mirroring those observed for the S220A variant (Supplementary Fig. 14).

Ar-X-Ar motifs are vital for specific binding to the SurA hotspots
To investigate the role of aromatic-containing motifs in SurA binding to the core and P1 binding sites, we produced an Ar-X-Ar-containing peptide from the sequence of a de novo designed transmembrane βbarrel (TMB), named MVK (TMB2.3β3) 82 .Despite TMB2.3 and SurA having no evolutionary history, we observed interactions of MVK (TMB2.3β3) with SurA-WT in both the core and P1 binding sites (Supplementary Fig. 16).These results provide additional supporting evidence that both the P1 and core binding sites recognise Ar-X-Ar motifs that are enriched in OMP sequences 57 .
We next substituted aromatic residues within the Ar-X-Ar motif (YRY) of the OmpX-derived QMN (β2) peptide (QMN YRY ) with Ala, both separately (QMN ARY , QMN YRA ), and together (QMN ARA ) (Supplementary Fig. 17, Supplementary Table 2).Peptides with a single Ala substitution removed the interaction with the core binding site and reduced peptide interaction at the P1 site (Supplementary Fig. 17a-f), while interactions with both sites were abolished by the double Ala substitution (Supplementary Fig. 17g, h).Finally, we recorded NMR spectra of SurA-WT in the presence of two truncated QMN (β2) peptides: one comprising the N-terminal eight residues of QMN (QMNKMGGF -QMN N-term ), which lacks an Ar-X-Ar motif, and one comprising the C-terminal seven residues, which contains the Ar-X-Ar motif (NLKYRYE -QMN C-term ).As expected, QMN N-term that contains no Ar-X-Ar motif, had little effect on the spectrum of SurA-WT, consistent with the importance of Ar-X-Ar in mediating affinity (Fig. 5a, b).By contrast, QMN C-term demonstrated binding at the core site, but with binding largely abolished at the P1 site (Fig. 5c, d).Intriguingly, the CSPs shown to be indicative of the core-P1 conformational change when full-length QMN (β2) binds (Supplementary Fig. 14) are absent for QMN C-term binding (Fig. 5d), suggesting that whilst Ar-X-Ar motifs mediate binding, their flanking sequences are also important for relaying information to SurA when bound.Taken together, these results highlight the crucial role of Ar-X-Ar motifs in OMP recognition at both binding hotspots, and highlight the different sequence specificities at the two binding sites.

Discussion
The OMP chaperone, SurA, plays a key role in cell envelope homoeostasis 33,34 and in promoting bacterial growth and virulence 47,83 .Yet, despite recent progress in mapping SurA domain dynamics (using smFRET 56 , SANS 53 , and NMR 55 ) and client binding (using cross-linking MS 54,56 , HDX-MS 56 , and SANS 54 ), the molecular details of how SurA binds its substrates and releases them to BAM for folding remain poorly understood.Here, we used methyl-TROSY NMR to map regions of the chaperone responsible for client recognition and binding in residue-specific detail.Previous models suggested that the affinity between the unfolded OMP client and SurA is mediated by diffuse binding to the chaperone surface involving the core/P1 (and potentially P2) domains 54,56 .In addition to the possibility of weak, diffuse binding, our NMR data, generated using different OMP sequences and OMP-derived peptides, revealed two OMP-binding hotspots, one located in the crevice between the two lobes of the N-domain of the core, and the other in the P1 domain.Differences (>100-fold) in affinity of short peptides derived from OmpX compared with the intact protein highlight the potential importance of both the binding hotpots and diffuse, weak interactions across the binding surface to enhance binding affinity.Further, from the perspective of the OMP client, we show that aromatic-containing motifs are important for OMP recognition at both sites, but that different patterns of aromatic residues and/or their flanking residues alter the specificity for each site.Ar-X-Ar motifs are enriched in OMPs 57 , and are often found at the membranewater interface in native OMP structures 6,7 .The specificity of SurA for these motifs helps explain how the chaperone can bind many different OMPs that exhibit a large diversity of sequence, size, and structures (Supplementary Fig. 7).
Recent AlphaFold-based predictions of the interaction between unfolded OmpA and SurA also suggested that clients bind in the core domain binding site we identified here 84 .These models suggested that a tyrosine (Y128) located at the base of the SurA core binding site forms π-π interactions with aromatic residues in OmpA.This prediction is consistent with the crucial importance of aromatic residues in the Fig. 3 | Dual binding hotspots in SurA mediate affinity for OMP substrates.a OmpX-derived peptides analysed here mapped onto the crystal structure of natively folded OmpX (PDB: 1QJ8 64 ).b OmpX sequence highlighting peptides analysed, coloured as in (a).Ar-X-Ar and Ar-Ar motifs are highlighted with yellow boxes.c Raw intensity change data and (d) Z-score plots of peak intensity ratios for SurA-WT binding to OmpX.Peaks which are broadened below the noise in the intensity ratio plots are indicated by an asterisk.For these peaks, the noise value for the bound spectra was used in the calculation of Z-scores.Signal to noise ratios were used for the calculation of errors in peak intensities (Methods).e Residues showing significant Z-scores for SurA-WT binding to OmpX mapped onto a model of SurA in a core-P1 open conformation.The 13 C-labelled δ1 and ε carbon atoms of Ile and Met residues, respectively, are shown as spheres coloured by Z-score (red for Z-scores > 0 and white for Z-scores <= 0).f Raw CSP data and (g) Z-score plots of CSPs for SurA-WT binding to the OmpX-derived KHD (β6) peptide, comprising a 15residue sequence that forms β-strand 6 in the OmpX native state.h Residues showing different CSP Z-scores for SurA-WT binding to the KHD (β6) peptide mapped onto a SurA core-P1-open model (coloured as in (g)).The effects of other OmpX-derived peptides on the SurA spectrum are shown in Supplementary Figs. 9, 10. i Model of SurA-WT in the core-P1 open state bound with peptides modelled in at the two binding hotspots.A short polypeptide sequence from a neighbouring molecule in the crystal structure (PDB: 1M5Y 48 ) (cyan) is located in the core domain binding crevice.A peptide identified by phage-display which binds to the SurA P1 domain (WEYIPNV) 58 (pink) is modelled in from the P1-WEYIPNV peptide crystal structure (PDB: 2PV1 59 ).Source data are provided as a Source Data file.
substrate for binding at this site, as we show via substitution of Tyr with Ala in the Ar-X-Ar motif of the OmpX-derived QMN peptide (Supplementary Fig. 17).The two binding hotspots may have different roles in chaperone function, for instance, promoting core-P1 opening, client expansion, client release and/or delivery to BAM.Additionally, by enabling different regions of the same unfolded OMP chain to anchor to each site, the two binding hotspots may facilitate vectorial delivery of unfolded OMPs to BAM for folding.Exactly how OMPs are released from SurA and delivered to BAM remain open questions, but changes in conformational dynamics of SurA on BAM binding 50 , together with the weak affinities of SurA-OMP interactions 49,81,85 , and ultimately the driving force of the onset of OMP folding into the OM 86 are all likely to play a role.
Combining the evidence presented, we propose a refined model for SurA:OMP chaperone activity (Fig. 6).In the absence of its OMP clients, SurA is found predominantly in its auto-inhibited core-P1 closed state 52,53 .Binding of unfolded OMPs via their aromatic motifs to the chaperone promotes opening of the core-P1 interface to create a more active state.Given that the core binding site is accessible in core-P1 closed models 48,53 , the results suggest that auto-inhibition of core activity by P1 occurs via suppression of conformational dynamics of the two lobes that comprise the core domain binding site, rather than  direct blocking of this site from OMP binding.Consistent with this, crosslinking P1 to the core domain leads to OMP assembly defects in vivo 52 and reduced crosslinking to OMP substrates in vitro 54 .Relief of the auto-inhibition of the core domain by P1 (in which the P2 domain may play a role 49,52 ) could involve increased flexibility and dynamics within the core domain's binding crevice, and/or clamp-like motions between the two lobes of the N-domain.Increased flexibility induced by mutations is known to enhance the activity of other ATPindependent chaperones, such as Spy 87 , and clamp-like binding sites are commonly found in both ATP-dependent (e.g., Hsp70, and Hsp90 families) 88 , and ATP-independent chaperones (e.g., Skp, prefoldin) 88,89 .Activation of ATP-independent chaperones often involves order-todisorder transitions and/or changes in oligomeric state 90,91 , while for ATP-dependent chaperones, activation depends upon structural rearrangements caused by the binding, hydrolysis and/or release of nucleotides 92,93 .By contrast, our data reveal that in the case of SurA, it is interaction with substrates which induces domain rearrangements that activate the chaperone.
Inhibition of SurA activity is a promising strategy for combating Gram-negative pathogen growth, virulence, and resistance to current antibiotics.However, given previous models based on diffuse binding to the chaperone surface, how this could be achieved was unclear.Our identification of two binding hotspots for OMP clients in the core and P1 domains reveal these sites as excellent potential targets for small molecules, which could potentially interfere simultaneously with chaperone binding, activation, substrate remodelling, and OMP delivery to BAM.

Bioinformatics analysis of SurA domain conservation
To assess the level of conservation of PPIase domains in SurA domain architectures, we obtained 14,244 SurA homologues from the InterPro database (InterPro family IPRO15391).We identified core only homologues in several steps.First, homologues were filtered by selecting those which had no predicted PPIase domain InterPro families (IPR046357, IPR000297, IPR023058, or IPR023034) annotated in their UniProt entries (1159 sequences).Next, we used Alpha-Fold structural predictions for these sequences to separate homologues which contain unannotated PPIase domains from genuine core only homologues.Of the 1159 sequences, 1120 predicted structures were found in the EBI-hosted AlphaFold database (https:// alphafold.ebi.ac.uk/) (AF_<uniport_id > _F1_model_v3.pdbfiles).We generated structural predictions for the remaining 39 homologues using Alphafold-Multimer (v2.1.0)installed on a local workstation and the reduced databases as described at https://github.com/deepmind/alphafold.We then used DSSP predictions of the secondary structure for filtering as, unlike the core domain, PPIase domains all have a topology that includes three consecutive β-strands.Successful filtering was manually confirmed by visualisation in PyMOL.By evaluating these AlphaFold2-generated structures we found that only 73/1159 of these sequences were predicted to be true core domain only homologues (Source Data file).The majority of these are from the γ-protobacterial class in the genus Halomonas (49/73).Therefore, only ~0.5% of the 14,244 SurA homologues in the InterPro database are predicted to be core domain only homologues (Fig. 1c).Analysis was carried out using in-house Python 3 scripts and made use of the Biopython (v1.79) 94,95 library.

Conservation analysis of SurA homologues
To obtain a set of SurA homologues with a three domain architecture, sequences with lengths between 400 and 450 residues were extracted from the InterPro SurA_N family (IPRO15391) 96 (8250/14,225 homologues).These sequences were then clustered with CD-HIT 97 using a 40% sequence identity threshold to give 303 representative sequences.A multiple sequence alignment (MSA) of these sequences was obtained with Clustal Omega 98  SurA-WT ± QMN N-term int.ratios SurA-WT ± QMN N-term CSPs SurA-WT ± QMN C-term int.ratios SurA-WT ± QMN C-term CSPs JalView v2.11.2.5 99 .This MSA was used to generate per residue conservation scores via the ConSurf webserver 100,101 .Residues with a ConSurf score of 7/9 or higher were considered highly conserved.

Plasmid constructs
The expression plasmid pET28b containing the mature SurA sequence (residues 21-428) preceded by an N-terminal 6x His-tag and thrombincleavage site (pSK257) 102 was a kind gift from Daniel Kahne (Harvard University, USA).The thrombin-cleavage site was changed to a TEVcleavage site using mutagenesis, yielding SurA with the N-terminal sequence MGSS(H) 6 SSGENLYFQG.SurA domain deletion variants SurA-ΔP1, SurA-ΔP2, and SurA-core were constructed by deleting codons for residues 172-280, 281-389 or 172-389, respectively, as described previously 49 .The expression plasmid for the mature sequence of tOmpA (residues 22-192) in pET11a 103 was kindly provided by Karen Fleming (John Hopkins University, USA).Codon-optimised synthetic genes (Eurofins) of the mature sequences of OmpX (residues 24-171) and OmpF (residues 23-362) were cloned into pET11a (Novagen) between the NdeI (5′) and BamHI (3′) restriction sites.pET29b+ expression plasmids encoding the Cys-OmpX-Cys, Cys-OmpX, and OmpX-Cys constructs used in smFRET experiments were obtained from TWIST bioscience.The Cys-OmpX-Cys construct contained the N-terminal sequence Met-Gly-Ser-Cys followed by the codonoptimised mature OmpX sequence (residues 24-171) and an additional C-terminal Cys residue 63 .The Cys-OmpX 50 and OmpX-Cys single Cys variants of the Cys-OmpX-Cys construct lacked the C-terminal and N-terminal Cys residues, respectively.The Cys-tBamA construct for use in MST experiments, was also obtained from TWIST bioscience in a pET29b+ expression vector.This construct contained the N-terminal sequence Met-Gly-Ser-Cys followed by the codon-optimised mature tBamA sequence (residues 425-810).The two natural Cys residues in tBamA were mutated to Ser (C690S/C700S).To create tBamA-pET11a, residues 425-810 of BamA were amplified by PCR, using plasmid BamAB-pETDUET-1 (kindly donated by Susan Buchanan (NIH, USA)) as the template, and the resultant product was then ligated into pET11a between NdeI and BamHI restriction sites.The plasmids containing SurA isoleucine and methionine point mutants (Supplementary Table 3) used in NMR peak assignment experiments were obtained from TWIST bioscience cloned into pET29b+.All mutagenesis was performed using Q5 site-directed mutagenesis (NEB).The protein sequences of expressed OMPs and SurA variants are provided in the Source Data file.Binding of OMP substrates (red) to the two identified binding hotspots in the core and P1 domains enables activation of the chaperone and leads to OMP expansion (left).These dual binding sites recognise aromatic containing motifs (e.g., Ar-X-Ar) that are enriched in OMP sequences 57 .Whether it is OMP binding to the core site, P1 site, or both, that leads to chaperone activation is currently not clear, though our results implicate P1 binding in this process (Fig. 4, Supplementary Fig. 13).Binding sites of both hotspots are accessible in the core-P1 closed state suggesting that auto-inhibition is due to repression of conformational dynamics of the core domain, rather than steric blocking, and that sequences linking binding motifs in intact unfolded OMP clients are important for chaperone activation.Expansion of the substrate and prevention of locally collapsed regions in the unfolded chain by activated SurA suggests a mechanism for delivery of the 'linearised' client to BAM for its vectorial folding into the OM 54,63 .

Labelling of Cys-OmpX-Cys variant
Purified Cys-OmpX-Cys was covalently labelled stochastically with Alexa Fluor 488 and ATTO 565 dyes via maleimide chemistry.A sample containing 423 μM Cys-OmpX-Cys in 25 mM Tris-HCl, 6 M Gdn-HCl, pH 7.2, was incubated with 10 mM DTT for 30 min.This sample was subsequently buffer exchanged into 25 mM Tris-HCl, 6 M Gdn-HCl, pH 7.2 (that had been sparged for 15 min with nitrogen gas) using Zeba spin desalting columns (Thermo Fisher Scientific).Alexa Fluor 488 C5 maleimide (Thermo Fisher Scientific) (10 mM dissolved in DMSO) and ATTO 565 maleimide (ATTO-TEC GmbH) (10 mM dissolved in DMSO) were immediately added to the OmpX sample to final concentrations of 635 µM and 1.17 mM, respectively.The total sample volume was 500 µL.The labelling reaction was incubated at 25 °C for 30 min only to avoid non-specific labelling.The reaction was quenched with 10 mM DTT and the sample then loaded onto a Superdex Peptide 10/300 column (GE Healthcare) equilibrated with 3 M Gdn-HCl, 25 mM Tris-HCl, pH 7.2 to remove the excess free dye.Samples were collected every 1 mL and peak protein fractions tested for dye labelling using a Nanodrop 2000 (Thermo Fisher Scientific).Samples containing double-labelled Cys-OmpX-Cys were snap-frozen using liquid nitrogen and stored at −80 °C until required.
For labelling of Cys-OmpX with ATTO 565 and OmpX-Cys with both Alexa Fluor 488 and ATTO 565, samples containing 300 µM protein were first incubated with 10 mM DTT for 30 min.Samples were then buffer exchanged into 25 mM Tris-HCl, 6 M Gdn-HCl, pH 7.2 (that had been sparged for 15 min with nitrogen gas) using Zeba spin desalting columns (Thermo Fisher Scientific).Either Alexa Fluor 488 C5 maleimide (Thermo Fisher Scientific) (10 mM dissolved in DMSO) or ATTO 565 maleimide (ATTO-TEC GmbH) (10 mM dissolved in DMSO) as appropriate were immediately added to final concentrations of 450 µM (1.5x excess) and incubated at 25 °C for 30 min.The reaction was quenched with 10 mM DTT, and the sample then loaded onto a Superdex Peptide 10/300 column (GE Healthcare) equilibrated with 3 M Gdn-HCl, 25 mM Tris-HCl, pH 7.2 to remove the excess free dye.Samples were collected every 1 mL and peak protein fractions tested for dye labelling using a Nanodrop 2000 (Thermo Fisher Scientific).

smFRET data collection
Samples were measured at a volume of 100 μL in an 18 well sample chamber (81817, Ibidi) with the focal spot 50 μm above the cover glass on a custom-built confocal microscope (Supplementary Methods) at room temperature (~21 °C).Laser powers measured before the objective lens of the two lasers used for donor and acceptor excitation were an average of 75 μW and 35 μW, respectively.smFRET of samples was measured at a concentration of ~75 pM OmpX in measurement buffer (20 mM Tris-HCl, 0.1 M urea, 0.02% (v/v) Tween-20, pH 8.0) with 1 mM aged Trolox ((±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid) to prevent photobleaching of the sample 104 .Aged Trolox was prepared by adding 1 mM of Trolox to measurement buffer and incubating the sample overnight at room temperature on a rotary shaker to dissolve before being filtered using a 0.22 μm pore size membrane.Tween-20 (0.02% v/v) was added to prevent non-specific absorption to the glass slide.In experiments monitoring FRET-labelled OmpX in the presence of SurA variants, a concentration of 30 μM SurA-WT or SurAcore was used.Each sample was freshly prepared and measured in 1 h acquisitions with a total of 5 measurements for apo-OmpX (0.1 M urea) and 6 measurements for OmpX + SurA-WT and OmpX + SurA-core.apo-OmpX in 4 M urea was measured for 1 h.

smFRET data analysis
The ptu files from the SymPhoTime64 software were converted into HDF5 data files using phconvert v0.9 (available at https://github.com/Photon-HDF5/phconvert).Each of these files was then merged to create one file for each condition.These merged files were then analysed using the FRETBursts v0.7.1 python package 105 .Firstly, the background was independently calculated for every 60 s period of measurement.Following the background calculation, a burst search was performed, selecting bursts with a minimum threshold of 3x the background signal in the donor and acceptor channels, a minimum total burst size of 50 photons in the donor and acceptor channels from the donor excitation.Bursts were then corrected 106 through the FRETBursts software 105 using the following values: donor leakage = 0.14, direct excitation = 0.14, gamma factor = 1.0 and beta factor = 1.4.Bursts were then filtered by removing events with an S > 0.7 and <0.3 and an ALEX-2CDE 107 filter with a cut off of 95 (as implemented in FRETBursts) to remove bursts with photobleaching, excessive photophysics or potential multi-molecule events.
BVA 68 was used to assay for dynamics on the ms timescale occurring during bursts.This analysis compares the expected variance in E raw based on shot noise against the measured variance in E raw in each individual burst (s i ).BVA was performed in the FRETbursts environment.To calculate s i , we segmented each burst into consecutive windows of 5 photons, and calculated the standard deviation of all windows within the burst.To help visualise the average s i at each E raw we segmented the E raw axis into bins with a width of 0.05.
The apparent fluorescence lifetime of the donor fluorophore in the presence of the acceptor (τ D(A) ) within each FRET burst was calculated by taking the mean microtime (corresponding to donor excitation and donor emission) and subtracting the mean microtime of the instrument response function 108 .The lifetime of the donor molecule in the absence of the acceptor (τ D(0) ) was calculated in the same fashion, but for OmpX molecules which were only labelled with a donor fluorophore (i.e., a S value >0.9).The static and dynamic FRET lines were calculated using the FRETlines python package version e099b1a (available at https://github.com/Fluorescence-Tools/FRETlines) 109.

Expression and purification of SurA variants for NMR spectroscopy
Plasmids containing wild-type SurA or SurA variant genes were transformed into BL21(DE3) cells (Stratagene).A daytime starter culture of 5 mL LB, supplemented with 40 µg/mL kanamycin, was grown from a single colony for ~6 h at 30 °C to an OD 600 of ~0.5.This culture was centrifuged, resuspended in 100% D 2 O and added to NMR media (Supplementary Table 5) supplemented with 40 µg/mL kanamycin, in 250 mL baffled conical flasks, for overnight growth at 30 °C.For a typical 100 mL culture the volume of the overnight culture was 24 mL.The following morning the remaining 76 mL of NMR media supplemented with 40 µg/mL kanamycin was added to the culture and grown at 37 °C until an OD 600 of ~0.5-6 was reached (~4-6 h).Reagents for Ile and Met labelling α-ketobutyric acid and L-methionine (Supplementary Table 6) at 70 mg/L and 250 mg/L were added and the culture grown for 1 h before expression was induced with the addition of 0.4 mM IPTG.After 6 h of expression the cells were harvested by centrifugation.We found that expression with 2x M9 minimal media (Supplementary Table 5) to increase buffering capacity led to an approximately 1.5-2-fold increase in yields 110 .Purification was performed as for unlabelled SurA with some variation in the protocol for the purification of Ile and Met variants for assignment.For the latter, all buffers were the same, but cells from these small-scale expression cultures (50 mL) were lysed by sonication, resuspended in 1 mL and His 6 SpinTrap columns (Cytivia) were used for the purification according to manufacturer's instructions.

NMR spectroscopy
All NMR experiments were performed on a 950-MHz Bruker Ascend Avance III HD NMR spectrometer equipped with either a Bruker 3 mm TCI or 5 mm TXO triple-resonance cryogenically cooled probe.Peptide binding experiments were performed in 5 mM EDTA, 20 mM Tris-HCl, pH 8.0 with 5 % (v/v) D 2 O at 25 °C.Binding experiments using fulllength OMP sequences also contained 0.8 M urea.Data was acquired for ~16 h with the exception of OMP-containing experiments which were acquired for ~5 h.Methyl-TROSY NMR experiments used a bandselective optimised-flip-angle short-transient experiment 112 (2D 1 H- 13 C SOFAST-HMQC) 70 .Pulse sequences were obtained using the NMRlib 113 plugin for TopSpin.Data was processed with NMRPipe 114 and analysed with CcpNmr Analysis v2.5 115 .Further analysis and all plotting was carried out using in-house Python 3 scripts using the Matplotlib (v3.2.2) 116 , NumPy (v1.18.5) 117 , Nmrglue (v0.8) 118 , and Biopython (v1.79) 94,95 libraries.Signal to noise ratios were used for the calculation of errors in peak intensities, with the noise floor obtained for each spectrum using NMRPipe (specStat.com) 114.Errors in the intensity ratios for each peak between two spectra were obtained by propagating the errors according to: where δR is the error in the intensity ratio, |R| is the value of the intensity ratio, X and Y are the intensity values for the peak in the first and second spectrum, respectively, and δX and δY are the noise floor values for the first and second spectrum, respectively.For the calculation of CSPs, the shifts in the proton and carbon dimensions were scaled according to the relative gyromagnetic ratios of the nuclei: where ΔH and ΔC are the changes in proton and carbon chemical shift, respectively.Z-scores for intensity ratio and CSP data were calculated using: where x i is the value for a particular peak, and µ and σ are the mean and standard deviation for all peaks, respectively.

Assignment of SurA Ile-Cδ1 and Met-Cε resonances in NMR spectra
For assignment of SurA-WT Ile and Met residues (43 in total) each residue was individually mutated (Supplementary Table 3), and the effect of the mutation assessed by methyl-TROSY NMR.Samples contained 5-70 µM SurA variant.Assignment experiments (2D 1 H- 13 C SOFAST-HMQCs) for Ile or Met variants were performed in 20 mM sodium phosphate, 50 mM NaCl, pH 7.5 (at 35 °C), or 20 mM Tris-HCl, pH 8 (at 25 °C), respectively.Two pairs of peaks (I70/I280, I100/I103) were overlapped.One pair of peaks in the P1 domain (I202/I259) could not be unambiguously assigned.One pair of severely broadened peaks in the core domain (M46/M400) could also not be unambiguously assigned.Further one peak in the P1 domain (I217) was also severely broadened and not included in analysis.Finally, the peak for I75 could not be separated from that for I202/I259 in many experiments, so it was also not included in analyses.Peaks for I70 and M400 appear in new locations in experiments in which core-P1 open states are populated (SurA-WT + WEYIPNV and SurA-S220A) or the P1 domain is deleted (SurA-core and SurA-ΔP1).These were assigned by performing experiments in which WEYIPNV (50 µM) was added to the I70V and M400A SurA variants.The 1 H chemical shift assignments were referenced using 2,2-dimethyl-2-silapentane-5-sulphonic acid (DSS) at 25 °C as a standard.The 13 C chemical shifts were referenced indirectly to DSS.NMR assignments have been deposited in the Biological Magnetic Resonance Bank (BMRB) with Entry ID 52180.

Peptide synthesis
Peptides from internal regions of OMPs were modified with N-terminal acetylation and C-terminal amidation and were either ordered from GenScript (USA) or produced in-house.All amino acids, Rink amide resin, N,N'-diisopropylcarbodiimide (DIC), 2cyano-2-(hydroxyimino)acetate (Oxyma), trifluoroacetic acid, triisopropylsilane and 2,2′-(Ethylenedioxy)diethanethiol (DODT) were purchased from Fluorochem or Merck.All amino acids were N-Fmoc protected and side chains were protected with either tBu (Asp, Glu, Ser, Thr, Tyr), Trt (Asn, Gln, Cys, His), Boc (Lys, Trp) or Pbf (Arg).Piperidine and acetic anhydride were purchased from Fisher.ACS grade N,N-dimethylformamide (DMF) and HPLC grade Acetonitrile from Merck were used during the synthesis and analysis.The identity of the peptide was confirmed by high-resolution mass spectrometry on Bruker Maxis Impact spectrometer using electrospray ionisation.The purity was determined on Agilent 1290 Infinity II system using Ascentis peptide column and gradient 5-95% of acetonitrile in water with 0.1% (v/v) trifluoroacetic acid additive at 0.5 ml/min for 10 min.The peptide synthesis was performed in microwave assisted Liberty Blue CEM peptide synthesiser equipped with HT loader at 0.1 mmol scale on Rink-amide resin (loading 0.48 mmol/g).Standard preprogramed coupling and deprotection cycles were applied.The deprotection was achieved using 20% (v/v) piperidine in DMF with microwave heating at 90 °C for 100 s, followed by three DMF washing steps.The couplings were performed using 5 eq. of Fmoc-protected amino acid, 5 eq.DI and 5 eq.Oxyma in DMF with microwave heating at 90 °C for 3 min followed by two DMF washing steps.The N-terminal acetylation was performed using 10% (v/v) acetic anhydride in DMF with microwave heating at 60 °C for 3 min followed by two DMF washing steps.The resin with synthesised peptide was then transferred to SPS tube, washed three times with 10 Ll dichloromethane, two times with 10 mL of diethylether and dried under vacuum for 30 min.To deprotect the side chains and cleave the peptide from the resin, the resin was incubated on a rotator for 3 h with 10 ml of cleavage mix (92.5% (v/v) trifluoroacetic acid, 2.5% (v/v) water, 2.5% (v/v) triisopropylsilane, 2.5% (v/v) DODT) and filtered.The filtrate was concentrated to ca. 1 mL under a stream of nitrogen and the peptide was precipitated by addition of 10 mL of ice-cold diethylether and isolated by centrifugation (4226 × g for 5 min).The precipitate was resuspended in 10 mL of ice-cold diethylether and isolated by repeating the centrifugation step.After decanting the diethylether, the precipitate was allowed to dry for 30 min, dissolved in 5Ll of 1% (v/v) acetic acid and freeze dried.

Peptide purification
Peptides were dissolved in 4-10 mL of 1:1 mixture of acetonitrile and water and purified using Agilent 1260 infinity system equipped with UV detector and fraction collector on Kinetex EVO 5 µm C18 100 Å 21.2 × 250 mm reverse phase column.1-4 mL of the peptide solution was injected and 25 min gradient of 10-40% (v/v) acetonitrile in water with 0.1% (v/v) formic acid additive was run at 10 ml/min.The fractions containing peptide were pooled and freeze dried.

Isothermal calorimetry (ITC)
ITC experiments were performed on a MicroCal iTC200 instrument in duplicate at 25 °C.The buffer used in all experiments was 20 mM Tris-HCl, pH 8.For binding experiments between SurA-WT and the peptide WEYIPNV, concentrations of 20 µM SurA-WT and 200 µM in the sample cell and syringe were used, respectively.For binding experiments between SurA-WT and OmpX-derived peptides QMN and KHD, concentrations of 100 µM SurA-WT and 1000 µM QMN/ KHD in the sample cell and syringe were used, respectively.Other Ar-X-Ar containing OmpX-derived peptides were not soluble at these concentrations.20 injections (1 × 0.5 µL and 19 × 2 µL) of peptides were added successively to the sample cell with a spacing of 150 s and a stirring rate of 750 rpm.Reference experiments in which SurA-WT was absent from the sample cell were performed and subtracted from the corresponding peptide binding data.The first data point was discarded in all experiments.Data were processed, analysed and fitted using OriginLab ITC v1.25.5.

Labelling of Cys-tBamA with Alexa Fluor 488
Purified Cys-tBamA was covalently labelled with Alexa Fluor 488 dye via maleimide chemistry.A sample containing 153 μM Cys-tBamA in 25 mM Tris-HCl, 6 M Gdn-HCl, pH 7.2, was incubated with 10 mM DTT for 30 min.This sample was subsequently buffer exchanged into 25 mM Tris-HCl, 6 M Gdn-HCl, pH 7.2 (that had been sparged for 15 min with nitrogen gas) using Zeba spin desalting columns (Thermo Fisher Scientific).Alexa Fluor 488 C5 maleimide (Thermo Fisher Scientific) (10 mg/mL dissolved in DMSO) was immediately added to the Cys-tBamA sample at a final concentration of 1.5 mM.The total sample volume was 260 µL.The labelling reaction was kept at 25 °C for 1 h then left overnight at 4 °C.The reaction was then loaded onto a Superdex Peptide 10/300 column (GE Healthcare) equilibrated with 6 M Gdn-HCl, 25 mM Tris-HCl, pH 7.2 to remove the excess free dye.Samples were collected every 1 mL and peak protein fractions tested for dye labelling using a Nanodrop 2000 (Thermo Fisher Scientific).Samples containing labelled tBamA were snap-frozen using liquid nitrogen and stored at −80 °C until required.

Microscale thermophoresis (MST)
Alexa Fluor 488-labelled tBamA or OmpX was buffer exchanged into 8 M urea and 20 mM Tris-HCl, pH 8.0.A stock of 200 μM SurA-WT or SurA-core in 20 mM Tris-HCl, pH 8.0 was used to create a serial dilution for tBamA (40 μM-1.2 nM) and for OmpX (12.5 μM-3 nM).A 1 µM stock of Alexa Fluor 488-labelled tBamA or OmpX was first diluted to 200 nM with 20 mM Tris-HCl, pH 8.0, then added to SurA variant samples to give final concentrations of 100 nM tBamA/OmpX and 0.8 M urea in 20 mM Tris-HCl, pH 8.0.The samples were loaded into premiumcoated capillaries (NanoTemper Technologies GmbH, Germany) and measured using a Monolith NT.115 (NanoTemper Tech.) at 25 °C.Data were fitted to a Hill equation: where S obs is the observed signal; S U and S B are the signal of the unbound and bound state, respectively; K d , app is the apparent K d ; L is the ligand, which in these experiments is SurA-WT or SurA-core, and n is the Hill coefficient.Fits and plots were made with in-house Python 3 scripts and made use of the SciPy (v1.5.0) and Matplotlib (v3.2.2) 116 libraries.

Reporting summary
Further information on research design is available in the Nature Portfolio Reporting Summary linked to this article.

Fig. 1 |
Fig.1| Structure, conformations and domain architecture of E. coli SurA. a Crystal structure of wild-type (WT) E. coli SurA (PDB: 1M5Y48 ) with missing residues added using MODELLER56,119 .In this structure the P1 domain is bound to the core domain (named here, SurA core-P1 closed).Regions are coloured grey (Nterminal region of the core domain), green (P1), yellow (P2) and orange (C-terminal region of the core domain).This colour scheme for SurA domains is used throughout.b Model of WT SurA in which the P1 domain is extended away from the core domain (named here SurA core-P1 open).The model was built using

Fig. 2 |
Fig. 2 | smFRET captures the conformational dynamics of unfolded OmpX in the presence or absence of SurA variants.a Schematic showing OmpX (black line) with donor and acceptor fluorophores on the N-and C-termini (green and red) with example alternate conformers shaded in grey (top), E raw histogram (middle) and BVA analysis (lower) for apo-OmpX.The solid black line represents the expected standard deviation for a static (ms timescale) FRET population.The white circles show the mean s i for bin widths of 0.05 of E raw .b OmpX + SurA-WT and (c) OmpX + SurA-core with panels as in (a).The schematic of SurA is coloured according to Fig. 1.Source data are provided as a Source Data file.

Fig. 5 |
Fig. 5 | Effects of truncation of the OmpX-derived QMN (b2) peptide on the methyl-TROSY NMR spectrum of SurA-WT.Intensity ratio (a, c) and CSP (b, d) plots are shown for SurA-WT bound to (a, b) the N-terminal 8 residues of QMN (QMN N-term -QMNKMGGF), and (c, d) the C-terminal 7 residues of QMN (QMN C-term -NLKYRYE).Peaks which were broadened beyond detection in peptide-containing samples are indicated with an asterisk.Conf.: residues indicative of conformational change (Supplementary Fig. 14).Samples contained 5 µM SurA ± 200 µM peptide, 5 mM EDTA, 20 mM Tris-HCl, pH 8, at 25 °C.int: intensity.Signal to noise ratios were used for the calculation of errors in peak intensities in (a, c) (Methods).Source data are provided as a Source Data file.

Fig. 6 |
Fig.6| Proposed activation mechanism for OMP binding to SurA.In the absence of substrate SurA is predominantly in an auto-inhibited core-P1 closed conformation (top) with a minor population (~25%53 ) of an active core-P1 open conformation (right).Binding of OMP substrates (red) to the two identified binding hotspots in the core and P1 domains enables activation of the chaperone and leads to OMP expansion (left).These dual binding sites recognise aromatic containing motifs (e.g., Ar-X-Ar) that are enriched in OMP sequences57 .Whether it is OMP binding to the core site, P1 site, or both, that leads to chaperone activation is currently not 59 with default settings accessed via Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made.The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material.If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.To view a copy of this licence, visit http://creativecommons.org/ licenses/by/4.0/.